西美杰代理的瑞典Agrisera成立于1980年,作为全球知名植物抗体研发生产商,致力于研发高品质植物单克隆及多克隆抗体。Agrisera公司拥有1000多种用于植物和藻类研究的一抗,主要集中于逆境、生理、病理等相关蛋白抗体。Agrisera一直为广大科研工作者提供热门研究领域的抗体,例如:Loadingcontrol抗体、Mitochondrial抗体、Photosynthesis抗体、Environmentalstress-related抗体、Rubisco抗体等。本篇文章小编将着重推荐Agrisera经典臻品Rubisco/Carbonmetabolism抗体。
1、Rubisco抗体
Rubisco(核酮糖-1,5-二磷酸羧化酶)是光合作用中决定碳同化速率的关键酶,同时也是植物光呼吸的关键酶。从紫色细菌到植物,它的同源性已经得到了证实,并且由两个蛋白质亚基组成,每个亚基有8个拷贝。在植物和绿藻中,大亚基(~55KDa)由叶绿体rbcl基因编码,小亚基(15kDa)由核rbcs基因家族编码。Agrisera的Rubisco相关抗体已验证可与多种植物和藻类有反应性,如拟南芥、芹菜、青蒿等。
部分产品信息:
货号 |
品名 |
实验类型 |
AS03 037 |
RbcL | Rubisco large subunit, form I (rabbit) |
IL、IF、IG、TP、WB |
AS03 037A |
RbcL | Rubisco large subunit, form I (affinity purified) |
WB |
AS03 037-HRP |
RbcL | Rubisco large subunit, form I, HRP-conjugated (40 µg) |
ELISA、WB |
AS15 2955 |
RbcL II | Rubisco large subunit, form II |
WB |
AS07 259 |
RbcS | Rubisco small subunit (SSU) |
WB |
AS07 259A |
RbcS | Rubisco small subunit (SSU) (affinity purified) |
ELISA、WB |
部分产品应用示例:
AS03037
AS152955
部分产品参考文献:
[1]Wang et al. (2020). Effects and Mechanisms of Foliar Application of Silicon and Selenium Composite Sols on Diminishing Cadmium and Lead Translocation and Affiliated Physiological and Biochemical Responses in Hybrid Rice (Oryza Sativa L.) Exposed to Cadmium and Lead. Chemosphere. 2020 Jul;251:126347. doi: 10.1016/j.chemosphere.2020.126347.
[2]Khajuria et al. (2020). Photochemical Efficiency Is Negatively Correlated With the Δ 9- Tetrahydrocannabinol Content in Cannabis Sativa L. Plant PhysiolBiochem. 2020 Apr 8;151:589-600. doi: 10.1016/j.plaphy.2020.04.003.
[3]Kurmayer et al. (2020). Chemically labeled toxins or bioactive peptides show a heterogeneous intracellular distribution and low spatial overlap with autofluorescence in bloom-forming cyanobacteria. Sci Rep. 2020 Feb 17;10(1):2781. doi: 10.1038/s41598-020-59381-w. (Immunofluorescence)
[4]Zhang et al. (2020). Hydrogen sulfide and rhizobia synergistically regulate nitrogen (N) assimilation and remobilization during N deficiency-induced senescence in soybean. Plant Cell Environ. 2020 Feb 3. doi: 10.1111/pce.13736.
[5]Zavřel et al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.
2、Carbonic anhydrase抗体
碳酸酐酶(Carbonicanhydrase,CA)是一种含锌金属酶。催化CO2和HCO3的可逆相互转化,它在许多涉及脱羧或羧化反应的生理功能中发挥重要作用,包括光合作用和呼吸作用。Agrisera的Carbonic anhydrase相关抗体已验证可与衣藻有反应性。
部分产品信息:
货号 |
品名 |
实验类型 |
AS05 073 |
CAH3 | Carbonic anhydrase |
IF、WB |
AS19 4322 |
CAH6 | Carbonic anhydrase 6 |
WB |
部分产品应用示例:
AS05073
部分产品参考文献:
[1]Terentyev (2020).The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..
[2]Terentyev et al. (2019). Carbonic anhydrase CAH3 supports the activity of photosystem II under increased pH.BiochimBiophys Acta Bioenerg. 2019 Jul 1;1860(7):582-590. doi: 10.1016/j.bbabio.2019.06.003.
3、Phosphoenolpyruvate carboxylase抗体
磷酸烯醇丙酮酸羧化酶(Phosphoenolpyruvate carboxylase,PEPC),EC=4.1.1.31,属于羧化酶家族。在PEPC催化下,CO2与磷酸烯醇丙酮酸(PEP)结合生成草酰乙酸。磷酸烯醇丙酮酸羧化酶(PEPC)是碳同化途径中最重要的酶之一,无论C3还是C4植物中都存在。
部分产品信息:
货号 |
品名 |
实验类型 |
AS09 458 |
PEPC | Phosphoenolpyruvate carboxylase |
IL、WB |
AS09 458-HRP |
PEPC | phosphoenolpyruvate carboxylase, HRP-conjugated (40 µg) |
WB |
AS09 458-ALP |
PEPC | phosphoenolpyruvate carboxylase, ALP-conjugated (40 µg) |
WB |
部分产品应用示例:
AS09458
部分产品参考文献:
[1]Durallet al. (2020). Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803. Biotechnol Biofuels. 2020 Jan 28;13:16. doi: 10.1186/s13068-020-1653-y.
[2]Wanget al. (2019). PUB25 and PUB26 Promote Plant Freezing Tolerance by Degrading the Cold Signaling Negative Regulator MYB15.
[3]Hui et al. (2018). TALE-carrying bacterial pathogens trap host nuclear import receptors for facilitation of infection of rice. Mol Plant Pathol. 2018 Nov 30. doi: 10.1111/mpp.12772.
4、Phosphoribulokinase抗体
磷酸核酮糖激酶(Phosphoribulokinase,PRK)是卡尔文循环的关键酶,消耗光反应过程中产生的ATP。Agrisera的Phosphoribulokinase抗体已验证可用于拟南芥、玉米、水稻等植物。
部分产品信息:
货号 |
品名 |
实验类型 |
AS07 257 |
PRKribulose-5-P-kinase | Phosphoribulokinase |
WB |
AS07 257-ALP |
PRK ribulose-5-P-kinase | phosphoribulokinase, ALP-conjugated (40 µg) |
WB |
AS07 257-HRP |
PRK ribulose-5-P-kinase | Phosphoribulokinase, HRP-conjugated (40 µg) |
WB |
部分产品应用示例:
AS07257
部分产品参考文献:
[1]Fukayamaet al. (2018). Expression level of Rubisco activase negatively correlates with Rubisco content in transgenic rice. Photosynth Res. 2018 May 30. doi: 10.1007/s11120-018-0525-9.
[2]Pérez-Ruiz et al. (2017). NTRC-dependent redox balance of 2-Cys peroxiredoxins is needed for optimal function of the photosynthetic apparatus. Proc Natl Acad Sci U S A. 2017 Nov 7;114(45):12069-12074. doi: 10.1073/pnas.1706003114.
除上述介绍外,Agrisera还为广大植物科研客户提供前沿研究所需的TRX、Lci5、SBP等高品质已验证抗体,更多产品信息获取可登陆西美杰官网:www.xmjsci.com;或拨打全国客服电话:400-050-4006咨询。
西美杰作为Agrisera中国区代理,为广大客户提供优质的已验证抗体和售前售后服务支持。同时西美杰整合全球高品质植物学品牌:PhytoTech和PlantCellTechnology;免疫学品牌:KPL、Lifespan、Cali-Bio,为科研客户提供一站式服务,解决科研客户选择困难、质量担忧等问题。